A simplified multiplex PCR assay for fast and easy discrimination of globally distributed staphylococcal cassette chromosome mec types in meticillin-resistant Staphylococcus aureus
نویسندگان
چکیده
منابع مشابه
Enhanced multiplex PCR assay for typing of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus.
We previously published a simple multiplex PCR assay for quick-screening of Staphylococcal Cassette Chromosome mec (SCCmec) types I-V, which has been extensively used worldwide. An updated assay is described here and the changes serve to make this rapid assay more accurate and reliable in facilitating identification of most common and major SCCmec types.
متن کاملNovel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus.
Staphylococcal cassette chromosome mec (SCCmec) typing is essential for understanding the molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA). SCCmec elements are currently classified into types I to V based on the nature of the mec and ccr gene complexes, and are further classified into subtypes according to their junkyard region DNA segments. Previously described trad...
متن کاملStaphylococcal Cassette Chromosome mec Types Among Methicillin-Resistant Staphylococcus aureus in Northern Iran
BACKGROUND Methicillin-resistant Staphylococcus aureus (MRSA) is a common cause of nosocomial and community-acquired infections around the world. Staphylococcal cassette chromosome mec (SCCmec) typing methods are often used to study MRSA molecular epidemiology. OBJECTIVES The current study was designed to explore the distribution profiles of different SCCmec types among methicillin-resistant ...
متن کاملstaphylococcal cassette chromosome mec types among methicillin-resistant staphylococcus aureus in northern iran
conclusions similarly to other geographical regions of iran, the sccmec type iii mrsa strain was the most frequently isolated strain from patients in gorgan. staphylococcal cassette chromosome mec type iii showed fewer virulence factors compared to other sccmec types. results the most common sccmec type was type iii, with a frequency rate of 76%, followed by types iv, i, and v, with frequency r...
متن کاملCharacterization of Staphylococcal Cassette Chromosome Mec Elements in Biofilm-producing Staphylococcus Aureus, Isolated from Hospital Infections in Isfahan
Background and Aims: Staphylococcus aureus is one of the important pathogens around the world. The present investigation was carried out to study the distribution of Staphylococcal Cassette Chromosome Mec (SCCmec) types and antibiotic resistance properties in methicillin-resistant Staphylococcus aureus isolated from Isfahan hospitals. Materials and Methods: A total of 250 clinical specimens ...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2010
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.021956-0