A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis

A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis

Improved Protocol for Isolation of Genomic DNA from Leaf Tissues of Phyllanthus emblica Gaertn

Modified Cetyltrimethylammonium bromide (CTAB) protocol for DNA isolation was developed from leaf tissuesof Phyllanthus emblica for obtaining high quality genomic DNA. Fresh leaves of three different maturitywere analyzed for yield and quality of DNA. Acidity was determined in three different maturity of leaves viz.tender, intermediate and mature and their influence on DNA qua...

An Efficient Protocol for Silkmoth Dna Extraction Suitable for Genomic Fingerprinting and Genetic Diversity Analysis

In the present study, fresh Silkmoths were used for DNA extraction. The wings, legs and antennae of moths were cut with sterile scissors, crushed under liquid nitrogen to fine powdered content which yielded high resolution DNA bands using modified phenol chloroform extraction method. The genomic DNA from twelve different silkmoth genotypes was further checked for its suitability in DNA fingerpr...

An Efficient Protocol for Genomic Dna Isolation from Cultivable Bacteria

A standardized protocol can play a major role in isolation of DNA especially when dealing with bacterial cultures. The aim of the present study was to develop a standard protocol of ultrapure high yield bacterial genomic DNA extraction from bacterial culture suitable for use in molecular biology. Extraction procedure was optimized with series of steps, which involved an unpublished modification...

Rapid DNA Extraction Protocol from Stool, Suitable for Molecular Genetic Diagnosis of Colon Cancer

Colorectal cancer (CRC) is one of the most common forms of cancers in the world and is curable if diagnosed at the early stage. Analysis of DNA extracted from stool specimens is a recent advantage to cancer diagnostics. Many protocols have been recommended for DNA extraction from stool, and almost allof them are difficult and time consuming, dealing with high amount of toxic materials like phen...