A standardized ZAP-70 assay—Lessons learned in the trenches

ZAP-70 methylation status is associated with ZAP-70 expression status in chronic lymphocytic leukemia.

BACKGROUND AND OBJECTIVES ZAP-70 expression is a recognized prognostic marker in chronic lymphocytic leukemia (CLL). The aim of this study was to analyze whether the methylation status of the ZAP-70 gene is associated with expression of the ZAP-70 protein. DESIGN AND METHODS Patients with CLL (n=87), acute lymphoblastic leukemia (n=13), mantle cell leukemia (n=13) and splenic marginal zone ly...

Differential Requirements for ZAP-70 in TCR

Reconstitution of T cell receptor signaling in ZAP-70-deficient cells by retroviral transduction of the ZAP-70 gene

A variant of severe combined immunodeficiency syndrome (SCID) with a selective inability to produce CD8 single positive T cells and a signal transduction defect in peripheral CD4+ cells has recently been shown to be the result of mutations in the ZAP-70 gene. T cell receptor (TCR) signaling requires the association of the ZAP-70 protein tyrosine kinase with the TCR complex. Human T cell leukemi...

Zap - 70 ( zeta - chain - associated protein 70 kD ) deficiency

A decade of arboviral activity—Lessons learned from the trenches

In the ten years during which PLOS Neglected Tropical Diseases has been publishing, it has become known as a critical journal for describing new and ground-breaking research in arbovirology. In its first year in existence, ten arboviral papers were published in this journal while last year, 157 arboviral papers were published here. In just the past decade, outbreaks of dengue, chikungunya, and ...