A STUDY OF SIEVE ELEMENT STARCH USING SEQUENTIAL ENZYMATIC DIGESTION AND ELECTRON MICROSCOPY

A Study of Sieve Element Starch Using Sequential Enzymatic Digestion and Electron Microscopy

The fine structure of plastids and their starch deposits in differentiating sieve elements was studied in bean (Phaseolus vulgaris L.). Ultrastructural cytochemistry employing two carbohydrases specific for different linkages was then used to compare the chemical nature of "sieve tube starch" (the starch deposited in sieve elements) with that of the ordinary starch of other cell types. Hypocoty...

Measurement of resistant starch by enzymatic digestion in starch and selected plant materials: collaborative study.

Interlaboratory performance statistics was determined for a method developed to measure the resistant starch (RS) content of selected plant food products and a range of commercial starch samples. Food materials examined contained RS (cooked kidney beans, green banana, and corn flakes) and commercial starches, most of which naturally contain, or were processed to yield, elevated RS levels. The m...

Localization of Dna and Protein in Tipula Iridescent Virus (tiv) by Enzymatic Digestion and Electron Microscopy

De-embedded ultrathin sections of ethanol-fixed Tipula Iridescent Virus particles were incubated with pepsin at pH 1.8, trypsin at pH 7.7, and DNase at pH 7.7. The outer shell of the particles, but not an inner core, was removed by the action of pepsin. Conversely, the inner core, but not the outer shell, was removed by the action of trypsin and DNase in combination, but not by either enzyme ac...

Study on hepatitis B virus infection using electron microscopy and gel diffusion techniques

preliminary study of in vivo formed dental plaque using confocal microscopy and scanning electron microscopy

objective: confocal laser scanning microscopy (clsm) is relatively a new light microscopical imaging technique with a wide range of applications in biological sciences. primary value of clsm for the biologist is its ability to provide optical sections from three-dimensional specimen. the present study was designed to assess the thickness content of in vivo accumulated dental plaque using clsm a...