A third unlinked gene controlling the pyruvate dehydrogenase complex in Aspergillus nidulans

Two unlinked genes for the pyruvate dehydrogenase complex in Aspergillus nidulans.

The activity of the overall pyruvate dehydrogenase complex was found to be similar in extracts of Aspergillus nidulans after growth on either sucrose or acetate. Eight mutants lacking the activity of this complex were found among some 200 glycolytic mutants selected for their inability to grow on sucrose. The absence of pyruvate dehydrogenase complex activity was also confirmed for a mutant, g6...

Mutation Analysis of the Pyruvate Dehydrogenase Exa Gene in Eight Patients With a Pyruvate Dehydrogenase Complex Deficiency

Mutation analysis of the pyruvate dehydrogenase E1 alpha gene in eight patients with a pyruvate dehydrogenase complex deficiency.

Most of the mutations causing deficiency of the pyruvate dehydrogenase (PDH) complex are in the X-linked E1 alpha gene. We have developed a rapid screening method for the detection of mutations in this gene using reverse transcription of total RNA, polymerase chain reaction amplification of the whole coding region of the gene and single-strand conformation polymorphism (SSCP) analysis. With thi...

Fine-structure mapping of the acetamidase structural gene and its controlling region in Aspergillus nidulans.

A large number of amdS mutants altered in acetamide utilization have been used to construct a fine-structure map of the amdS locus. The mutagen diepoxyoctane generated most of the deletion strains used for mapping. A minimum of 14 sites within the amdS gene were found. Biochemical analysis of amdS mutants defined the extent of the probable coding region. A new mutant, amd-205, which did not pro...

Pyruvate dehydrogenase kinase activity of pig heart pyruvate dehydrogenase (E1 component of pyruvate dehydrogenase complex).

The pyruvate dehydrogenase (E1) and acetyltransferase (E2) components of pig heart and ox kidney pyruvate dehydrogenase (PDH) complex were separated and purified. The E1 component was phosphorylated (alpha-chain) and inactivated by MgATP. Phosphorylation was mainly confined to site 1. Addition of E2 accelerated phosphorylation of all three sites in E1 alpha and inactivation of E1. On the basis ...