A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7

Multiplexed, real-time PCR for quantitative detection of human adenovirus.

Adenovirus infection is becoming increasingly recognized as a cause of morbidity and mortality in the immunosuppressed patient population. While early detection and quantitation of adenovirus in peripheral blood has been suggested as a means of directing and monitoring antiviral therapy in these patients, few methods have been published, particularly with respect to viral quantitation. A multip...

Development of a Sensitive Quantitative Competitive PCR Assay for Detection of Human Cytomegalovirus DNA

Accurate and rapid diagnosis of human cytomegalovirus (HCMV) disease in immunocompromised patients has remained as a challenge. Quantitative competitive PCR (QC-PCR) methods for detection of HCMV in these individuals have improved the positive and negative predictive values of PCR for diagnosis of HCMV disease. In this study we used QC-PCR assay, using a co-amplified DNA standard, to quantitate...

An internally controlled , triplex quantitative PCR assay 7 for human polyomaviruses JC and BK 8 Triplex quantitative PCR assay for JC and BK polyomaviruses 9

Quantitative real-time PCR assay for detection of Ehrlichia chaffeensis.

A real-time PCR assay was developed for the detection of Ehrlichia chaffeensis. The assay is species specific and provides quantitative results in the range 10 to 10(10) gene copies. The assay is not inhibited by the presence of tick, human, or mouse DNA and is compatible with high sample throughput. The assay was compared with previously described assays for E. chaffeensis.

Development of a TaqMan Real-Time PCR Assay for the Rapid Detection of Bovine Adenovirus Serotypes in Cattle

The aim of this work was to develop a sensitive and specific real time PCR diagnostic assay for the rapid detection and identification of bovine adenoviruses (BAdVs) in cattle. A TaqMan real time PCR assay, capable of simultaneous detection of nine out of ten BAdV serotypes (species) belonging to the genera Atadenovirus and Mastadenovirus, was developed. The oligonucleotide primers and probe we...