A Two-Locus Global DNA Barcode for Land Plants: The Coding rbcL Gene Complements the Non-Coding trnH-psbA Spacer Region

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A Two-Locus Global DNA Barcode for Land Plants: The Coding rbcL Gene Complements the Non-Coding trnH-psbA Spacer Region

BACKGROUND A useful DNA barcode requires sufficient sequence variation to distinguish between species and ease of application across a broad range of taxa. Discovery of a DNA barcode for land plants has been limited by intrinsically lower rates of sequence evolution in plant genomes than that observed in animals. This low rate has complicated the trade-off in finding a locus that is universal a...

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Medical Archives and Manuscripts News, 2005

Background. A useful DNA barcode requires sufficient sequence variation to distinguish between species and ease of application across a broad range of taxa. Discovery of a DNA barcode for land plants has been limited by intrinsically lower rates of sequence evolution in plant genomes than that observed in animals. This low rate has complicated the trade-off in finding a locus that is universal ...

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A DNA barcode for land plants.

DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF-atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 g...

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discrimination of the invasive plant species, myriophyllum spp., from native relatives using dna barcoding

this study included 81 samples which belonged to 13 speices from myriophyllum genus. both nrdna its1 and its2 and cpdna matk, rbcl and trnh-psba loci were used for diagnose of invasive from native plant. the nrdna its1and its2 data proved highly variable and could differentiate between all but had low pcr amplification success,based on these result they would not recommend as a suitable barcode...

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Species identification of medicinal pteridophytes by a DNA barcode marker, the chloroplast psbA-trnH intergenic region.

Medicinal pteridophytes are an important group used in traditional Chinese medicine; however, there is no simple and universal way to differentiate various species of this group by morphological traits. A novel technology termed "DNA barcoding" could discriminate species by a standard DNA sequence with universal primers and sufficient variation. To determine whether DNA barcoding would be effec...

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ژورنال

عنوان ژورنال: PLoS ONE

سال: 2007

ISSN: 1932-6203

DOI: 10.1371/journal.pone.0000508