A Validated UPLC-MS/MS Method for Rapid Quantification of Umifenovir in Plasma Samples and Its Greenness Assessment

Umifenovir is one of the most often prescribed antiviral medications for prevention and treatment COVID-19 other viral infections. Herein, a UPLC-MS/MS method developed through using ibrutinib as an internal standard (IS) quantifying umifenovir in plasma samples. Both IS were analytically separated on Acquity BEH C18 column with total run time only 2.5 min. At flow rate 0.3 mLmin−1, acetonitrile:15 mM ammonium acetate (80:20) was employed mobile phase composition. Electrospray ionization positive mode used Detection quantification performed multiple reaction monitoring parent-to-daughter 477.05 → 279.02 441.16 84.4 IS, respectively. The validated following international guidelines bioanalytical validation, all parameters within acceptable limits. Moreover, eco-scale AGREE software evaluation greenness, results showed that very environmentally friendly. assay successfully bioavailability assessment newly formulation kneaded ternary umifenovir/β-cyclodextrin 1% poloxamer 188 (KDB).