Accurate quantification of mouse mitochondrial DNA without co-amplification of nuclear mitochondrial insertion sequences
نویسندگان
چکیده
منابع مشابه
Analysis of mitochondrial DNA sequences of Turcinoemacheilus genus (Nemacheilidae Cypriniformes) in Iran
Members of Nemacheilidae Family, Turcinoemacheilus genus were subjected to molecular phylogenetic analysis in this study. This genus was reported in 2009 to inhabit in Karoon River drainage, in contrary to previous assumption that it was the endemic species in the Basin of Tigris River. It was sampled from three stations placed in different tributaries in Karoon drainage and evaluated to unders...
متن کاملAnalysis of mitochondrial DNA sequences of Turcinoemacheilus genus (Nemacheilidae Cypriniformes) in Iran
Members of Nemacheilidae Family, Turcinoemacheilus genus were subjected to molecular phylogenetic analysis in this study. This genus was reported in 2009 to inhabit in Karoon River drainage, in contrary to previous assumption that it was the endemic species in the Basin of Tigris River. It was sampled from three stations placed in different tributaries in Karoon drainage and evaluated to unders...
متن کاملNuclear and mitochondrial DNA quantification of various forensic materials.
Due to the different types and quality of forensic evidence materials, their DNA content can vary substantially, and particularly low quantities can impact the results in an identification analysis. In this study, the quantity of mitochondrial and nuclear DNA was determined in a variety of materials using a previously described real-time PCR method. DNA quantification in the roots and distal se...
متن کاملNuclear and mitochondrial DNA sequences from two Denisovan individuals.
Denisovans, a sister group of Neandertals, have been described on the basis of a nuclear genome sequence from a finger phalanx (Denisova 3) found in Denisova Cave in the Altai Mountains. The only other Denisovan specimen described to date is a molar (Denisova 4) found at the same site. This tooth carries a mtDNA sequence similar to that of Denisova 3. Here we present nuclear DNA sequences from ...
متن کاملQuantification of mitochondrial DNA mutation load
Mitochondrial DNA (mtDNA) mutations are an important cause of genetic disease and have been proposed to play a role in the ageing process. Quantification of total mtDNA mutation load in ageing tissues is difficult as mutational events are rare in a background of wild-type molecules, and detection of individual mutated molecules is beyond the sensitivity of most sequencing based techniques. The ...
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ژورنال
عنوان ژورنال: Mitochondrion
سال: 2016
ISSN: 1567-7249
DOI: 10.1016/j.mito.2016.05.003