AcePrimer: automation of PCR primer design based on gene structure
نویسندگان
چکیده
منابع مشابه
VizPrimer: a web server for visualized PCR primer design based on known gene structure
SUMMARY The visualization of gene structure plays an important role in polymerase chain reaction (PCR) primer design, especially for eukaryotic genes with a number of splice variants that users need to distinguish between via PCR. Here, we describe a visualized web server for primer design named VizPrimer. It utilizes the new information technology (IT) tools, HTML5 to display gene structure an...
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While the amount of molecular data that can be routinely generated continues to increase, advances in the methods of analysis to handle this quantity of data have not kept pace. One area of research which could greatly benefit from new approaches is the design of specific probes and PCR primers. Here we generated spanning networks (1) for phytopathogenic Pseudomonas strains from seven species, ...
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Their large size and their relative resistance to proteolytic cleavage (1) make myosins particularly difficult substrates for the acquisition of their peptide sequences by standard protocols. For this reason, instead of identifying myosins first according to their biochemical activity and then obtaining their sequences, PCR and other DNA-based techniques exploiting the highly conserved sequence...
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Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given...
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ژورنال
عنوان ژورنال: Bioinformatics
سال: 2002
ISSN: 1367-4803,1460-2059
DOI: 10.1093/bioinformatics/18.11.1538