ACID-BASE PROPERTIES OF RHODOPSIN AND OPSIN

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Acid-base Properties of Rhodopsin and Opsin

Purified preparations of cattle rhodopsin have been titrated to various pH, irradiated, and the pH changes followed thereafter until completed. In this way we have obtained the titration curves of rhodopsin, of the immediate product of irradiation, measured within 30 seconds; and of the final product of irradiation (opsin). The rhodopsin preparations display about 54 titratable groups per mole ...

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The Stability of Rhodopsin and Opsin

THE STABILITY OF CATTLE RHODOPSIN AND OF ITS PROTEIN MOIETY OPSIN TOWARD ACIDS AND ALKALIES AND ON AGING WAS DETERMINED BY TWO CRITERIA: maintenance of absorption spectrum, and capacity to regenerate after exposure to light. On storage at 3 degrees C. at pH near neutrality, the absorption spectrum in the visible region may remain unchanged for as long as 6 months; but the regenerability progres...

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some properties of fuzzy hilbert spaces and norm of operators

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15 صفحه اول

Tiie Thermal Stability of Rhodopsin and Opsin by Ruth Hubbard*

Rhodopsin, the red photosensitive pigment of rod vision, is composed of a specific cis isomer of retinene, neo-b (ll-c~s), joined as chromophore to a colorless protein, opsin. We have investigated the thermal denaturation of cattle rhodopsin and opsin in aqueous digitonin solution, and in isolated rod outer limbs. Both rhodopsin and opsin are more stable in rods than in solution. In solution as...

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 1956

ISSN: 1540-7748,0022-1295

DOI: 10.1085/jgp.39.6.909