Activation of different Cl currents in Xenopus oocytes by Ca liberated from stores and by capacitative Ca influx.
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منابع مشابه
Activation of different Cl currents in Xenopus oocytes by Ca liberated from stores and by capacitative Ca influx
Xenopus oocytes are an excellent model system for studying Ca signaling. The purpose of this study was to characterize in detail the Ca-activated Cl currents evoked by injection of inositol 1,4,5-trisphosphate (IP3) into Xenopus oocytes voltage-clamped with two microelectrodes. Injection of IP3 into Xenopus oocytes activates two different Ca-activated Cl currents. ICl-1 is stimulated rapidly (w...
متن کاملCa(2+)-dependent activation of Cl(-) currents in Xenopus oocytes is modulated by voltage.
Ca(2+)-activated Cl(-) currents (I(Cl,Ca)) were examined using fluorescence confocal microscopy to monitor intracellular Ca(2+) liberation evoked by flash photolysis of caged inositol 1,4, 5-trisphosphate (InsP(3)) in voltage-clamped Xenopus oocytes. Currents at +40 mV exhibited a steep dependence on InsP(3) concentration ([InsP(3)]), whereas currents at -140 mV exhibited a higher threshold and...
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Ca2+-activated Cl- channels play important diverse roles from fast block to polyspermy to olfactory transduction, but their molecular identity has not been firmly established. By searching sequence databases with the M2 pore domain of ligand-gated anion channels, we identified potential Ca2+-activated Cl- channels, which included members of the bestrophin family. We cloned two bestrophins from ...
متن کاملAsymmetrical distribution of Ca-activated Cl channels in Xenopus oocytes.
Xenopus oocytes are a popular model system for studying Ca signaling. They endogenously express two kinds of Ca-activated Cl currents, I(Cl-1), and I(Cl-2). I(Cl-1) is activated by Ca released from internal stores and, with appropriate voltage protocols, by Ca influx. In contrast, I(Cl-2) activation is dependent on Ca influx. We are interested in understanding how these two different Cl channel...
متن کاملCa channels induced in Xenopus oocytes by rat brain mRNA.
RNA was isolated from brains of 16-d-old rats and poly(A) samples were injected into stage V and VI oocytes. After allowing 2-5 d for expression, most oocytes were exposed to medium in which the K had been replaced by Cs for 24 hr prior to recording. Ba currents were usually measured in Cl-free Ba-methanesulfonate saline. IBa in noninjected oocytes was often undetectable, but ranged up to 50 nA...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1996
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.108.3.157