Activation of human blood coagulation factor XI independent of factor XII.
نویسندگان
چکیده
منابع مشابه
Human blood coagulation factor XI. Purification, properties, and mechanism of activation by activated factor XII.
Human blood coagulation Factor XI was purified in 20% yield from plasma by ion exchange chromatography. The specific clotting activity of purified Factor XI was 250 & 20 units/mg implying that its concentration in normal titrated human plasma is 4 pg/ml. The purified Factor XI in its precursor form gave a single protein band on polyacrylamide gels in the presence of sodium dodecyl sulfate with ...
متن کاملActivation of Human Blood Coagulation Factor XI Independent of Factor XI1
Human blood coagulation factor XI was activated by either autoactivation or thrombin. These reactions occurred only in the presence of negatively charged materials, such as dextran sulfate (-Mr 500,000), sulfatide, and heparin. During the activation, factor XI was cleaved at a single Arg-Ile bond by thrombin or factor XIa to produce an amino-terminal 50-kDa heavy chain and a carboxyl-terminal 3...
متن کاملCharacterization of the Human Blood Coagulation Factor XI 1 Gene
A human genomic phage library was screened using a human factor XI1 cDNA as a hybridization probe. Two overlapping phage clones were isolated which contain the entire human factor XI1 gene. DNA sequence and restriction enzyme analysis of the clones indicate that the gene is approximately 12 kilobase pairs in size and is comprised of 13 introns and 14 exons. Exons 3-14 are contained in a genomic...
متن کاملRole of surface in surface-dependent activation of Hageman factor (blood coagulation factor XII).
The mechanism by which negatively charged substances such as celite, kaolin, or ellagic acid contribute to the surface-dependent activation of Hageman factor (Factor XII) was studied. Kinetic studies of the proteolytic activation of (125)I-labeled human Hageman factor by human plasma kallikrein, plasma, activated Factor XI, and trypsin were performed in the presence and absence of high molecula...
متن کاملContributions of human platelets to the proteolytic activation of blood coagulation factors XII and XI.
Previous studies have suggested that human platelets can promote the activation of factor XI by two different mechanisms, one requiring factor XII and ADP-treated platelets and the other requiring collagen-treated platelets in the apparent absence of factor XII. To investigate these hypotheses. isolated platelets were tested for their capacity to promote the activation and cleavage of purified ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1991
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)89453-8