Additive manufacturing of polymeric scaffolds for biomimetic cell membrane engineering

Additive manufacturing based on direct laser writing two-photon polymerization facilitates the fabrication of microstructures with full 3D design freedom. Here, this technique is exploited for engineering scaffolds accurately mimicking shape and size three types human cells. The cell models employed in study were chosen to include a range dimensions different identifiable features highlight versatility approach, yet other shapes can easily be fabricated similar manner. parameters additive process optimized obtain polymeric biomimetic shapes. After fabrication, converted polymer-cushioned model membranes through layer-by-layer functionalization cationic polymer lipid bilayer. Scaffold was verified using confocal scanning microscopy. Polymer-cushioned supported cells are particularly suitable membrane interaction studies where curvature plays an important role. aim demonstrate by high-resolution combined surface functionalization. interdisciplinary approach highlights value as technological solution challenges encountered biomedical studies.