Adsorption of polyacrylic acid on self-assembled monolayers investigated by single-molecule force spectroscopy
نویسندگان
چکیده
منابع مشابه
A Simple Bioconjugate Attachment Protocol for Use in Single Molecule Force Spectroscopy Experiments Based on Mixed Self-Assembled Monolayers
Single molecule force spectroscopy is a technique that can be used to probe the interaction force between individual biomolecular species. We focus our attention on the tip and sample coupling chemistry, which is crucial to these experiments. We utilised a novel approach of mixed self-assembled monolayers of alkanethiols in conjunction with a heterobifunctional crosslinker. The effectiveness of...
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The mechanical properties of poly(acrylic acid) (PAA) were investigated on the molecular scale by using AFM. PAA was adsorbed onto a glass substrate and then picked up at one point of the chain with the AFM tip and stretched. The deformation of single PAA chains under tension was measured and modeled by a modified freely-joint-chain model. The length distribution of loops and tails of adsorbed ...
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We report on a novel technique to nucleate nanometer-sized droplets on a solid substrate and to image them with minimal perturbation by noncontact atomic force microscopy (NC-AFM). The drop size can be accurately controlled, thus permitting hysteresis measurements. We have studied the nanoscale wettability of several methyl-terminated substrates prepared by the self-assembly of organic molecule...
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In typical force spectroscopy experiments, a small biomolecule is attached to a soft polymer linker that is pulled with a relatively large bead or cantilever. At constant force, the total extension stochastically changes between two (or more) values, indicating that the biomolecule undergoes transitions between two (or several) conformational states. In this paper, we consider the influence of ...
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ژورنال
عنوان ژورنال: New Journal of Physics
سال: 2004
ISSN: 1367-2630
DOI: 10.1088/1367-2630/6/1/009