Affinity chromatography of pig heart fumarase
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چکیده
منابع مشابه
Affinity chromatography of pig heart fumarase.
2-(5'-Phenylpentyl)fumaric acid was shown to be a competitive inhibitor (Ki 0.5 mM) of pig heart fumarase. After nitration of the aromatic ring, reduction to the amine and diazotization, the acid was attached via azo linkages to a Sepharose 4B-tyramine matrix. The resulting adsorbent was used for the affinity chromatography of crude fumarase, purifications of approx. 20-fold being obtained by s...
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Mersalyl is a powerful inhibitor of phosphate transport in mitochondria. In addition, by its reversible reaction with thiol groups, it can protect the latter from irreversible reaction with N-ethylmaleimide [ 1,2]. Use of this property enabled a group of proteins of M, 3.0-3.2 X lo4 to be identified in the internal mitochondrial membrane [3-g]. This group of proteins could be directly labeled u...
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متن کاملPig heart fumarase really does exhibit negative kinetic co-operativity at a constant ionic strength.
The kinetics of the action of fumarase on L-malate and fumarate were investigated at constant ionic strength. This was done to evaluate reports that fumarase follows simple Michaelis-Menten kinetics. However, when pH, buffer concentration and ionic strength are all maintained at constant values, the Lineweaver-Burk plots exhibit pronounced downward curvature, characteristic of negative kinetic ...
متن کاملpreparation of plasminogen by affinity chromatography
background: plasminogen is one of the compounds derived from human plasma. activation of plasminogen produces plasmin. plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. the aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine sepharose. materials and methods: normal human plasma was used as the start...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1979
ISSN: 0264-6021
DOI: 10.1042/bj1770115