Affinity chromatography on columns containing nucleic acids

Hydrophobic affinity chromatography of nucleic acids and proteins.

5' tritylated oligonucleotides binding hydrophobically to low trityl cellulose/sepharose (< 15 microMTr/ml) retain their hydrogen-bonding specificities for complementary sequences. This, constitutes a novel mode of attaching affinity ligands to solid supports, is more convenient than existing methods, and proceeds with 100% yield. The salt, dielectric constant and temperature dependence of thes...

A Generalized Design for Affinity Chromatography Columns

Lectin purification on affinity columns containing reductively aminated disaccharides.

A method is described for isolating lectins in pure form and quantitative yield in a single step by affinity chromatography on aminoethyl polyacrylamide gels containing reductively aminated disaccharide residues. The affinity columns were prepared in two steps: (a) direct reductive amination of the disaccharide and aminoethyl gel with sodium cyanoborohydride in aqueous solution at pH 9; (b) N-a...

Affinity chromatography with use of immobilized lectin columns.

360 It would be impossible to cover even briefly the distinguished work accomplished by Kobata and his colleagues during this period. Suffice to say the laboratory published no fewer than 128 full papers during the period 1983-1993, covering the glycobiology of many glycoproteins of biological or therapeutical interest. Two outstanding contributions are the demonstration of a change in IgG glyc...

Recent advances in high-performance liquid affinity chromatography columns.

High-performance liquid affinity chromatography (h.p.1.a.c.) is the term applied to the application of h.p.1.c. to affinity chromatography (Ohlson et al., 1978). Until recently affinity chromatography supports were unsuitable for use in high-performace applications. It is only in the last 8 years that these problems have been overcome, resulting in a second generation of affinity systems. The a...