Affinity purification of hydra glutathione bindings proteins
نویسندگان
چکیده
منابع مشابه
Affinity Based Nano-Magnetic Particles for Purification of Recombinant Proteins in Form of Inclusion Body
Background: Protein purification is the most complicated issue in the downstream processes of recombinant protein production; therefore, improved selective purification methods are important. Affinity-based protein purification method using His-tag and Ni-NTA resins is one of the most common strategies. MNPs can be used as a beneficial alternative for Ni-NTA resins. However, there is no data on...
متن کاملIn-house preparation of hydrogels for batch affinity purification of glutathione S-transferase tagged recombinant proteins
BACKGROUND Many branches of biomedical research find use for pure recombinant proteins for direct application or to study other molecules and pathways. Glutathione affinity purification is commonly used to isolate and purify glutathione S-transferase (GST)-tagged fusion proteins from total cellular proteins in lysates. Although GST affinity materials are commercially available as glutathione im...
متن کاملAzaserine Affinity Labelling of 7-glutamyl Transferase of Hydra Attenuata without Inactivation of the Glutathione Receptor
Intrigued by similar specificities of the hydra feeding receptor and y-glutamyl transferase activity toward GSH, we examined the possibility that these two GSH-bihding activities might reside in the same protein. We find that the two activities differ in specificity toward the y-glutamyl moiety of GSH. The hydra transferase recognizes L-azaserine, L-Glu, D-Glu and L-Gln. The feeding receptor re...
متن کاملPurification of sequence-specific DNA-binding proteins by affinity chromatography.
The affinity chromatography procedure described in this unit uses DNA containing specific recognition sites for the desired protein that has been covalently linked to a solid support. Preparation of a DNA affinity resin, including cyanogen bromide (CNBr) activation of the agarose support, is described, and an alternate protocol provides a method to couple DNA to commercially available CNBr-acti...
متن کاملBespoke affinity ligands for the purification of therapeutic proteins
2015 This article provides an overview of the current challenges and trends in bioprocessing, with a focus on recent advances in the affinity purification of the principal classes of biotherapeutics, including monoclonal antibodies, glycoproteins, vaccines and erythropoietin. Affinity chromatography is usually applied during large-scale protein purification; it involves affinity ligands of biol...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1995
ISSN: 0014-5793
DOI: 10.1016/0014-5793(95)98128-6