ALTERNATIVE FEED RESOURCES FOR FORMULATING CONCENTRATE DIETS OF RABBITS. 2. JACK BEAN (Canavalia ensiformis) SEEDS.
نویسندگان
چکیده
منابع مشابه
The Globulins of the Jack Bean, Canavalia Ensiformis
While working with the urease of the jack bean, the author has had occasion to study the nature of the proteins that are present. The proteins of the jack bean have been investigated by Jones and Johns,’ who claim to have isolated an albumin and two globulins. The precipitate obtained by half saturation with ammonium sulfate, present in small amount and having the greater sulfur content, they c...
متن کاملCloning, expression, and crystallization of jack bean (Canavalia ensiformis) canavalin.
Canavalin is the major storage protein of the jack bean (Canavalia ensiformis) and belongs to the classical vicilin fraction. A full-length cDNA for canavalin was generated by the polymerase chain reaction. The nucleotide sequence coding for canavalin and the corresponding amino acid sequence were determined and shown to be homologous with those of other seed storage proteins. The amino acid se...
متن کاملPurification and Properties of Urease Derived from Hydrated Seeds of Jack Bean, Canavalia ensiformis (L) DC.
Urease from jack bean meal and hydrated seeds has been obtained in 25 to 33% yield with specific activity in the range of 1000 to 1070 units/mg protein. A purification of 100 to 130-fold was achieved from meal and fully soaked seeds. Use of beta-mercaptoethanol and EDTA was found essential to obtain this high yield and purity. Amino acid analysis showed all 18 amino acids commonly found in prot...
متن کاملImmunofluorescent localization of urease in the cotyledons of jack bean, Canavalia ensiformis.
Urease has been localized in sections of cotyledons from germinating seeds of jack bean, using FITC-labelled immunoglobulin prepared from urease antiserum raised in rabbits. The complication of lectin binding to the immunoglobulins was resolved by treatment of the sections with specific glycosides. Urease is localized in 2 sites: within the cytoplasm of storage parenchyma cells in spherical gra...
متن کاملUltrastructural Effects of Water Stress on Chloroplast Development in Jack Bean (Canavalia ensiformis [L.] DC).
An ultrastructural study has been made to determine to what degree chloroplast differentiation is retarded in leaves of young jack bean (Canavalia ensiformis [L.] DC.) seedlings when they are subjected to mild water stress. Rapid chloroplast differentiation occurred when etiolated seedlings were allowed to green at relative humidities above 85% but not at a relative humidity of 25%. Response to...
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ژورنال
عنوان ژورنال: World Rabbit Science
سال: 2010
ISSN: 1989-8886,1257-5011
DOI: 10.4995/wrs.2000.429