Ambiguities in results obtained with 2D gel replicon mapping techniques
نویسندگان
چکیده
منابع مشابه
Ambiguities in results obtained with 2D gel replicon mapping techniques.
Recently, two 2-dimensional (2D) gel techniques, termed neutral/neutral and neutral/alkaline, have been developed and employed to map replication origins in eukaryotic plasmids and chromosomal DNA (1-11). The neutral/neutral technique, which requires less DNA for analysis, has been preferentially used in recent studies. We show here that the signal predicted for an origin is not detected using ...
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The nature of mammalian origins of DNA replication remains controversial and this is primarily because two-dimensional gel replicon mapping techniques have identified broad zones of replication initiation whereas several other techniques, such as quantitative PCR, have disclosed more discrete sites of initiation at the same chromosomal loci. In this report we analyze the replication of an ampli...
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چکیده ندارد.
15 صفحه اولcomparison of the obtained results from two calibration methods in mri brachytherapy normoxic polymer gel dosimetry
introduction: despite the fact that the clinical implementation of polymer gel dosimetry has been facilitated after the introduction of normoxic gels, there are still complications in its clinical routine use that are under investigation. in the current work, the feasibility of using a normoxic polymer gel dosimeter named magica has been investigated for use in our clinical brachytherapy applic...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.3.647