An efficient experiment for sequential backbone assignment of medium-sized isotopically enriched proteins
نویسندگان
چکیده
منابع مشابه
High resolution 4D HPCH experiment for sequential assignment of 13C-labeled RNAs via phosphodiester backbone
The three-dimensional structure determination of RNAs by NMR spectroscopy requires sequential resonance assignment, often hampered by assignment ambiguities and limited dispersion of (1)H and (13)C chemical shifts, especially of C4'/H4'. Here we present a novel through-bond 4D HPCH NMR experiment involving phosphate backbone where C4'-H4' correlations are resolved along the (1)H3'-(31)P spectra...
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Sequence specific resonance assignment is the prerequisite for the NMR-based analysis of the conformational ensembles and their underlying dynamics of intrinsically disordered proteins. However, rapid solvent exchange in intrinsically disordered proteins often complicates assignment strategies based on HN-detection. Here we present a six-dimensional alpha proton detection-based automated projec...
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چکیده ندارد.
Mars -- robust automatic backbone assignment of proteins.
MARS a program for robust automatic backbone assignment of (13)C/(15)N labeled proteins is presented. MARS does not require tight thresholds for establishing sequential connectivity or detailed adjustment of these thresholds and it can work with a wide variety of NMR experiments. Using only (13)C(alpha)/(13)C(beta) connectivity information, MARS allows automatic, error-free assignment of 96% of...
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ژورنال
عنوان ژورنال: Journal of Magnetic Resonance (1969)
سال: 1992
ISSN: 0022-2364
DOI: 10.1016/0022-2364(92)90169-8