An Enzymatic Colorimetric Quantification of Orthophosphate

Colorimetric vs enzymatic determination of serum phosphorus.

Enzymatic colorimetric assay of inorganic phosphate.

Vol.33, No.2, 1987 in June 1986, after recahibration of the glucose method, gave results equal to the survey mean: 640 and 1110 mg/L, respectively. The addition of two more calibration points solved our problem. However, we do not want to imply that five or more calibration points are required. The important question is not how many calibration points are used, but rather, are the concentration...

Homogeneous enzymatic colorimetric assay for total cysteine.

Patients with vascular disease have significantly higher concentrations of plasma total cysteine (tCYS) than do healthy individuals (1–7). The present method is a new, rapid, and sensitive enzymatic colorimetric assay for tCYS in plasma samples and is homogeneous in that it avoids separation methods. The tCYS assay uses only the recombinant enzymes methionine , -lyase (rMETase) and Sadenosylhom...

New enzymatic colorimetric assay for total homocysteine.

References 1. Linder MW, Prough RA, Valdes R. Pharmacogenetics: a laboratory tool for optimizing therapeutic efficacy. Clin Chem 1997;43:254–66. 2. Raimundo S, Fischer J, Eichelbaum M, Griese EU, Schwab M, Zanger UM. Elucidation of the genetic basis of the common ‘intermediate metabolizer’ phenotype for drug oxidation by CYP2D6. Pharmacogenetics 2000;10:577– 81. 3. Sachse C, Brockmoller J, Baue...

New Enzymatic Colorimetric Method for the Quantitative Determination of Phenylalanine in Dry-Blood Spots

Phenylketonuria (PKU) is an autosomal recessive disorder, which is characterized by severe mental retardation, microcephaly and seizures. The symptoms of this disease can be prevented if detected soon after birth. Therefore, blood Phenylalanine (Phe) measurement is essential for the early diagnosis, treatment and dietary monitoring of PKU patients. The goal of this research was to introduce a r...