An improved method to determine serine palmitoyltransferase activity
نویسندگان
چکیده
منابع مشابه
An improved method to determine serine palmitoyltransferase activity.
Serine palmitoyltransferase (SPT) catalyzes the condensation of l-serine and palmitoyl-CoA, which is the rate-limiting step in the de novo synthesis of sphingolipids. SPT activity is commonly measured by monitoring the incorporation of radiolabeled l-serine into 3-ketodihydrosphingosine. In this article, we introduce several adaptations of the established protocol to improve sensitivity, reprod...
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l h e activity of serine palmitoyltransferase (palmitoyl-coenzyme A [CoA]:i-serine [Ser]-C-palmitoyltransferase [decarboxylating], EC 2.3.1.50), the enzyme catalyzing the first step in the synthesis of the long-chain base required for sphingolipid assembly, has been characterized in a plant system. Enzyme activity in a microsoma1 membrane fraction from summer squash fruit (Cucurbita pepo 1. cv ...
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Serine palmitoyltransferase (SPT) catalyses the first step in the de novo biosynthesis of sphingolipids (SLs). It uses a decarboxylative Claisen-like condensation reaction to couple L-serine with palmitoyl-CoA to generate a long-chain base product, 3-ketodihydrosphingosine. SLs are produced by mammals, plants, yeast, and some bacteria, and we have exploited the complete genome sequence of Sphin...
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ژورنال
عنوان ژورنال: Chemistry and Physics of Lipids
سال: 2009
ISSN: 0009-3084
DOI: 10.1016/j.chemphyslip.2009.06.064