An Improved Radioisotope Dilution Assay for Serum Vitamin B12 Using Hemoglobin-coated Charcoal

An improved radioisotope dilution assay for serum vitamin B12 using hemoglobin-coated charcoal.

A radioisotope dilution assay for vitamin B12, using hemoglobin-coated charcoal, was modified in several respects, the most prominent of which was the use of cyanide extraction of sera. With these modifications, the B12 levels were significantly higher in sera from normal subjects and patients with folate deficiency, whereas sera from patients with pernicious anemia and other B12-deficient stat...

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An Improved Radioisotope Dilution Assay for Serum Vitamin B 2 Using Hemoglobin - coated Charcoal

A radioisotope dilution assay for vitamin B12, using hemoglobin-coated charcoal, was modified in several respects, the most prominent of which was the use of cyanide extraction of sera. With these modifications, the B12 levels were significantly higher in sera from normal subjects and patients with folate deficiency, whereas sera from patients with pernicious anemia and other B12-deficient stat...

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Measurement of Serum Vitamin B12 Level Using Radioisotope Dilution and Coated Charcoal.

I N PREVIOUS REPORTS’3 it was shown that uncoated charcoal adsorbs both free vitamin B12 and B12 bound to proteins, but charcoal precoated by mixing with saturating quantities of albumin adsorbs only free B12. This finding has been confirmed.4 The charcoal particles may be considered as solid microsponges and the albumin coat as a molecular sieve surrounding each sponge. Small molecules such as...

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