An In-solution Ultrasonication-assisted Digestion Method for Improved Extracellular Matrix Proteome Coverage
نویسندگان
چکیده
منابع مشابه
An in-solution ultrasonication-assisted digestion method for improved extracellular matrix proteome coverage.
Epithelial cell behavior is coordinated by the composition of the surrounding extracellular matrix (ECM); thus ECM protein identification is critical for understanding normal biology and disease states. Proteomic analyses of ECM proteins have been hindered by the insoluble and digestion-resistant nature of ECM. Here we explore the utility of combining rapid ultrasonication- and surfactant-assis...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2009
ISSN: 1535-9476
DOI: 10.1074/mcp.m900039-mcp200