An Optical Urate Biosensor Based on Urate Oxidase and Long-Lifetime Metalloporphyrins

Urate oxidase: primary structure and evolutionary implications.

Urate oxidase, or uricase (EC 1.7.3.3), is a peroxisomal enzyme that catalyzes the oxidation of uric acid to allantoin in most mammals. In humans and certain other primates, however, the enzyme has been lost by some unknown mechanism. To identify the molecular basis for this loss, urate oxidase cDNA clones were isolated from pig, mouse, and baboon, and their DNA sequences were determined. The m...

Efficacy of urate oxidase (uricozyme) in tumour lysis induced urate nephropathy.

Urate oxidase (uricozyme) is an enzyme of non-human origin capable of oxidizing human uric acid to allantoin, a highly soluble product at renal tubule pH. We report its efficacy in three patients with acute urate nephropathy due to tumour lysis in chronic lymphatic leukaemia and high grade lymphoma. Two patients had an additional obstructive nephropathy due to ureteric urate crystals. An intrav...

optimization of bacterial urate oxidase enzyme activity

Purification and characterization of extracellular Pseudomonas aeruginosa urate oxidase enzyme.

Urate oxidase (uricase) was isolated and purified from Pseudomonas aeruginosa to apparent homogeneity using ammonium sulphate precipitation followed by ion exchange and gel filtration chromatography. The specific activity of the purified uricase enzyme was found to be 636.36 with the use of uric acid as a substrate. The purified uricase enzyme is a monomeric protein with molecular weight of 64 ...

Thiouric acids as substrates and inhibitors of mammalian urate oxidase.

In spite of numerous investigations, the intricate mechanism of the oxidative degradation of uric acid by urate oxidase (urate-oxygen oxidoreductase, EC 1.7.3.3) has not been completely elucidated. The liver enzyme is a copper-protein complex (Mahler, Huebscher & Baum, 1955) which is activated by cysteine (Scheer & Scheer, 1943; Santagati & Topi, 1953). Cyanide, thiocyanate and hydroxylamine in...