Analysis of hepatitis B virus genotypes by restriction fragment length polymorphism

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Analysis of hepatitis B virus genotypes by restriction fragment length polymorphism.

INTRODUCTION Ten viral genotypes (A-J) distributed in all continents have been described for hepatitis B virus (HBV). One of the methodologies for determining the viral genotype is the restriction fragment length polymorphism (RFLP) technique, a simple and relatively inexpensive method, albeit with some limitations. OBJECTIVE The initial objective of the project was to identify the HBV genoty...

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A novel hepatitis B virus genotyping system by using restriction fragment length polymorphism patterns of S gene amplicons.

AIM Traditional hepatitis B virus (HBV) genotyping methods using restriction fragment length polymorphism (RFLP) can reliably identify genotypes A to F. As HBV genotypes G and H have been recently identified, this study was to establish an accurate and simple genotyping method for all eight HBV genotypes (A to H). METHODS Two hundred and forty HBV small S sequences obtained from GeneBank were...

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ژورنال

عنوان ژورنال: Biomédica

سال: 2015

ISSN: 0120-4157,0120-4157

DOI: 10.7705/biomedica.v36i0.2976