Analysis of multiplex PCR fragments with PMMA microchip
Multiplex PCR with multichannel microchip electrophoresis: an ultrafast analysis for genetic diseases.
A Y chromosomal polymorphic markers screening strategy using a multiplex polymerase chain reaction (PCR) and DNA microchip electrophoresis technology has recently been developed. It is a part of the human Y chromosome haplotyping system for studying Japanese population genetics and its relationship with male spermatogenic failure. This strategy is based on optimizing and modifying the primer se...متن کامل
We have developed a highly sensitive method for DNA analysis on 3D gel element microarrays, a technique we call multiplex microarray-enhanced PCR (MME-PCR). Two amplification strategies are carried out simultaneously in the reaction chamber: on or within gel elements, and in bulk solution over the gel element array. MME-PCR is initiated by multiple complex primers containing gene-specific, forw...متن کامل
An integrated continuous-flow microfluidic chip was fabricated on glass substrate with PDMS based microchannels, cell lysis and PCR modules. Gold-microelectrodes were used to produce electrochemical cell lysis, while, indium-tin-oxide (ITO) microheater was used for thermal cycling of PCR reaction. The fabricated device was used for 20 cycles of PCR amplification of pancreatic cancer DNA marker ...متن کامل
BACKGROUND approximately 20% of classic familial adenomatous polyposis (FAP) cases and 70% to 80% of attenuated FAP (AFAP) cases are negative for the APC/MUTYH point mutation. Quantitative multiplex PCR of short fluorescent fragments (QMPSF), a technique for detecting copy number alterations, has been successfully applied to several cancer syndrome genes. We used QMPSF for the APC gene to scree...متن کامل
Ultrafast diagnosis of the genetic-related disorders using the combined technologies of multiplex PCR and multichannel microchip electrophoresis.
For the diagnosis of unexplained male infertility a multiplex PCR for 6 markers, which are well-known as candidate genes for studying male infertility and located on the human Y-chromosome, has been designed. The multiplex PCR products have been separated on a 12 channel microchip electrophoresis system, which can analyze different samples simultaneously. By combining the technologies of multip...متن کامل
عنوان ژورنال: Talanta