Androgenic response of spring barley (Hordeum vulgare L.) varieties and lines – sources of high grain protein content

Aim. Protein content is known to be one of the most important characters grain quality. Investigation was aimed determine capability haploid production in anther culture vitro spring barley accessions which are sources high protein grain. Methods. Infrared spectroscopy used for evaluation variability genotypes content. Anthers isolated from cold-pretreated spikes were cultivated on agar solidified nutrient medium containing N6 macro-and MS micronutrients, physiologically active substances and maltose (9.0 %). Results. Three years testing showed that mean contents six varied 12.6 19.1 %. Commercial variety Yavir, IR06605 DD-21 germplasm collection National Plant Gene Pool Ukraine selected as with a stable expression this char-acter. Response androgenesis 6.9 45.9 % morphogenic anthers 0.64 до 14.7 green plants per 100 dependence genotype donor plant growth conditions. Conclusions. Because association between grains relatively androgenic response, varieties Velikan Yavir considered valuable ones use breeding improvement via vitro.