Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura of Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria

Abstract. Nasri, Harahap U, Silalahi J, Satria D. 2021. Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria. Biodiversitas 22: 3098-3104. Diarrhea is the discharge liquid or watery stools 3 to 4 times a day caused by bacterial infection. Treatments for diarrhea are probiotics, which have beneficial effect on health host such as antibacterial. Traditional Batak Toba fermented food, Naniura, source probiotics. This study aimed determine minimum inhibitory concentration, bactericidal and leakage DNA protein pathogens. Isolation LAB was obtained pour plate method deMann Rogosa Sharpe Agar + CaCO3 1%. In this study, Characterization analysis sequencing used Polymerase Chain Reaction. Determination MIC agar diffusion method. The MBC test streaking stroke inhibition zone formed. measured using spectrophotometry UV-VIS (260nm 280nm). isolation results were Lactobacillus fermentum, characterization showed that Gram-positive, bacilli, non-sporing, catalase-negative, able ferment sugar. determination at concentration 10%v/v with clear diameter. pathogens different concentrations. an increased absorption