Antibody engineering and phage display technology

Antibody engineering and phage display technology

Antibodies are an essential biotechnological tool being a part of immunodiagnostics, therapeutics and life science research instruments. Due to the advances of molecular biology methods, it became possible to manipulate structure and properties of antigen-binding domains of antibodies (1). This led to the creation of a variety of small-sized, single-domain, bi-specific, conjugated antibodies, a...

Antibody Engineering: Advances in Phage Display Technology and in the Production of Therapeutic Immunocytokines

Antibody phage display: technique and applications.

inTrOducTiOn The production of human monoclonal mAbs for research and clinical use is closely related to the development of phage display technology, initially described by Smith in 1985 and further developed by other groups (e.g., Winter, McCafferty, Lerner, Barbas). Antibody phage display (APD) is based on genetic engineering of bacteriophages (viruses that infect bacteria) and repeated round...

Phage display technology - Applications and innovations

The expression of exogenous peptides on the surface of filamentous bacteriophage was initially described by Smith in 1985. Since his first study, different molecules such as small peptides and antibodies have been displayed on coat proteins of phage, greatly expanding the applications of the technology. The past decade has seen considerable progress in the techniques and applications of phage l...

Phage display of combinatorial antibody libraries.

The selection of antibodies from combinatorial libraries displayed on the surface of filamentous phage has become an important methodology for the generation of reagent, diagnostic, and therapeutic molecules and for the study of natural immune responses. Using this technique, antibody genes have been cloned from multiple species or expressed directly from large man-made repertoires of antibody-...