Antimicrobial efficacy of Kerr pulp canal sealer (EWT) in combination with 10% amoxicillin on Enterococcus faecalis: A confocal laser scanning microscopic study

Background: Sealers with antimicrobial properties play an important role in endodontic therapy success especially against Enterococcus faecalis infection found failed root canal therapy. Addition of antibiotic agents to sealers may show significant increase their antibacterial both anaerobic and aerobic microbes. The purpose the present study was evaluate efficacy Kerr pulp sealer (EWT) combination 10% amoxicillin E. post-root treatment viability faecalis on first seventh day. Methods: A total 60 extracted human mandibular premolar teeth were decoronated after initial decontamination 1% NaOCl. Root length standardized 12 mm. Canal instrumentation done using ProTaper Universal file system till size F2 5.25% It then infected a pure strain for period four days. Obturation plain sealer, (n=30) sealer-antibiotic combination, (n=30). Half sectioned at 24 hours (S, SA) other half seven days obturation (S7, SA7). All samples stained SYTO9 propidium iodide imaging under Confocal Laser Scanning microscope. Statistical analysis performed statistical software SPSS v. 17.0 (SPSS Windows; Inc, Chicago, IL). Data analysed One Way ANOVA post hoc Tukey test determine significance p value < 0.01 considered significant. Results: Statistically differences observed green red ratio between group S (9.561976) S7 (0.435418) (p 0.01). There no difference SA (mean ratio, (0.70431) SA7 0.85184). Conclusions: Antibiotics added effectively eradicated post-obturation. However, days, as effective combination.