Antimicrobial Peptides Grafted onto a Plasma Polymer Interlayer Platform: Performance upon Extended Bacterial Challenge

To combat infections on biomedical devices, antimicrobial coatings have attracted considerable attention, including comprising naturally occurring peptides (AMPs). In this study the aim was to explore performance upon extended challenge by bacteria growing in media above samples. The AMPs LL37, Magainin 2, and Parasin 1 were selected basis of well-known membrane disruption activity solution covalently grafted onto a plasma polymer platform, which enables application multilayer coating strategy wide range biomaterials. Detailed surface analyses performed verify intended outcomes sequence. Samples challenged incubation bacterial growth for 5 20 h. Compared with control surface, all three AMP showed reductions colonization even at high initial seeding × 107 CFU, but there increasing numbers dead attached surface. All found be non-toxic primary fibroblasts. These thus could useful produce antibacterial biomaterials, though possible consequences arising from presence need studied further, compared non-fouling that avoid attachment bacteria.