Application of a Simple Multiplex PCR To Aid in Routine Work of the Mycobacterium Reference Laboratory
نویسندگان
چکیده
منابع مشابه
Application of a simple multiplex PCR to aid in routine work of the mycobacterium reference laboratory.
A PCR specific for spacer regions 33 and 34 of the direct repeat region of the Mycobacterium tuberculosis complex was developed to complement the biochemical differentiation of M. tuberculosis, Mycobacterium bovis, M. bovis BCG, and Mycobacterium africanum subtypes I and II. In addition, this approach was incorporated into a multiplex PCR that included primers specific for IS6110 and the 65-kDa...
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15 صفحه اول[Evaluation of a multiplex PCR assay to differentiate mycobacteria of the Mycobacterium tuberculosis complex in a reference laboratory].
Mycobacteria that cause tuberculosis in animals and humans belong to the Mycobacterium tuberculosis complex. Techniques for conventional diagnosis are time-consuming and do not differentiate between different strains belonging to the M. tuberculosis complex. The aim of this study was to evaluate a multiplex PCR assay applicable to mycobacteria in culture with the capacity to differentiate diffe...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2001
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.39.11.4166-4168.2001