Application of the FISH Method for Analyzing Chromosome Segregation Patterns in Preimplantation Embryos from Robertsonian Translocation Carriers

Robertsonian translocations (RTs) are very common balanced structural chromosome rearrangements in humans. Due to alterations the segregation pattern and formation of unbalanced gametes embryos, carriers RTs have a significant risk reproductive failure. For over 30 years, fluorescent situ hybridization (FISH) has been used for preimplantation genetic testing chromosomal rearrangement (PGT-SR) RTs. The data obtained by performing FISH PGT-SR can be useful analyzing patterns embryos. We analyzed 964 embryos from 100 couples who were various types or using array comparative genomic (aCGH) method. When male, predominant mode was alternate (detected 42.4% embryos), whereas, when female, adjacent detected most (32.3% 26.6% respectively). About 33% balanced/euploid IVF cycles formed RT about 24% such female. No association found between sperm parameters malsegregation rates male RT. In this study, we that female different risks associated with rate