Applying Multi-Response Optimization to Explore Fermentation Conditions of Probiotics

This review serves two purposes: first, to promote the use of improved optimization techniques in response surface methodology (RSM); and second, enhance optimum conditions for fermentation probiotics. According research dairy science, Lactiplantibacillus plantarum K79 is a candidate probiotic that has beneficial health effects, such as lowering blood pressure. The L. produce peptides with angiotensin-converting enzyme (ACE) inhibitory activity were proposed, through modeling each ACE pH function four factors are skim milk concentration (%), incubation temperature (°C), time (hours), starter added amount (%). To estimate conditions, researchers employed desirability-based multi-response approach, utilizing third-order models nonsignificant lack fit. estimated follows: 10.76%, an 36.9°C, 23.76 hours, 0.098%. Under these predicted was 91.047%, 4.6. These values achieved objectives this study.