Arabidopsis N6-methyladenosine reader CPSF30-L recognizes FUE signals to control polyadenylation site choice in liquid-like nuclear bodies

The biological functions of the epitranscriptomic modification N6-methyladenosine (m6A) in plants are not fully understood. CPSF30-L is a predominant isoform polyadenylation factor CPSF30 and consists CPSF30-S an m6A-binding YTH domain. Little known about roles molecular mechanism underlying its function alternative polyadenylation. Here, we characterized as Arabidopsis m6A reader whose required for floral transition abscisic acid (ABA) response. We found that activity enhances formation liquid-like nuclear bodies, where mainly recognizes m6A-modified far-upstream elements to control site choice. Deficiency lengthens 3? untranslated region three phenotypes-related transcripts, thereby accelerating their mRNA degradation leading late flowering ABA hypersensitivity. Collectively, this study uncovers new m6A-driven phase separation plants.