Assignment of YAC Clones Spanning Rice Chromosomes 10 and 12
نویسندگان
چکیده
منابع مشابه
Assignment of YAC clones spanning rice chromosomes 10 and 12.
Yeast artificial chromosome (YAC) clones were assigned on rice (Oryza saliva L. cv. Nipponbare) chromosomes 10 and 12 using DNA markers from our high-density linkage map. Out of 1,383 markers localized in this genetic map, 68 and 74 markers were located on chromosomes 10 and 12, respectively. Screening of the YAC genomic library was conducted by colony hybridization and Southern hybridization u...
متن کاملPhysical mapping of rice chromosomes 4 and 7 using YAC clones.
Physical maps of rice chromosomes 4 and 7 were constructed by landing yeast artificial chromosomes (YACs) along our high-density molecular linkage map. Using 114 DNA markers, 258 individual YACs were located on chromosome 4. Sixty-two out of 258 YACs carried two or more DNA marker positions and formed 16 contigs which covered a total length of 17.1 cM. The other YACs were arranged to 23 positio...
متن کاملPhysical mapping of rice chromosomes 8 and 9 with YAC clones.
First efforts for physical mapping of rice chromosomes 8 and 9 were carried out by ordering YAC clones of a rice genomic DNA library covering six genome equivalents with mapped DNA markers. A total of 79 and 74 markers from chromosomes 8 and 9, respectively, were analyzed by YAC colony and Southern hybridization using RFLP markers of cDNA and genomic clones, and by polymerase chain reaction (PC...
متن کاملOrdered YAC clone contigs assigned to rice chromosomes 3 and 11.
Yeast artificial chromosome (YAC) clones were arranged on the positions of restriction fragment length polymorphism (RFLP) and sequence-tagged site (STS) markers already mapped on the high-resolution genetic maps of rice chromosomes 3 and 11. From a total of 416 and 242 YAC clones selected by colony/Southern hybridization and polymerase chain reaction (PCR) analysis, 238 and 135 YAC clones were...
متن کاملGrowth and storage of YAC clones in Hogness Freezing Medium.
To date, frozen storage of YAC libraries have relied on the administration of glycerol to the medium subsequent to cell growth. By adding Hogness Freezing Medium prior to inoculation, cultures can be frozen directly after cell growth, with no adverse effect on the stability of the YAC DNA or on the viability of the cells even after repeated freezing and defrosting. Although a relatively simple ...
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ژورنال
عنوان ژورنال: DNA Research
سال: 1996
ISSN: 1340-2838
DOI: 10.1093/dnares/3.6.401