At-Line Reversed Phase Liquid Chromatography for In-Process Monitoring of Inclusion Body Solubilization

EVALUATION OF THE USE OF ANIONIC/ NONIONIC MIXED MICELLES IN REVERSED PHASE LIQUID CHROMATOGRAPHY OF CHLOROPHENOLS

The use of aqueous mixed micellar system consisting of sodium dodecyl sulfate (SDS) and polyoxyethylene (23) dodecanol (Brij-35) as the mobile phase in reversed-phase liquid chromatography was studied. A group of chlorophenols was used as the test mixture. Adding organic modifier to the system showed that the use of low concentrations of organic additives improves efficiency in SDS/Brij-35 mixe...

Reversed-phase high-performance liquid chromatography.

Micro-scale method for theophylline in body fluids by reversed-phase, high-pressure liquid chromatography.

We describe a micro-scale method for determining serum theophylline. The chromatography system includes a muBondapack C18 column and acetonitrile, 70 ml/liter of sodium acetate buffer (10 mmol/liter, ph 4.0) as the mobile phase. Test serum or plasma, 30 mul, is mixed with an equal quantity of a solution containing the internal standard, beta-hydroxyethyltheophylline in acetonitrile/sodium aceta...

Determination of monosaccharides in cider by reversed-phase liquid chromatography

A reversed-phase high-performance liquid chromatographic (HPLC) method is described for the simultaneous determination of aldoses and uronic acids in cider, previously derivatized with p-aminobenzoic ethyl ester (ABEE). Narrow-bore C8 columns are recommended as this alternative provides good separation efficiency, along with greater economy and sensitivity. Detection limits for aldoses (glucose...

Disposable reversed-phase chromatography columns for improved detection of carboxylic acids in body fluids by electron-capture gas-liquid chromatography.

Disposable reversed-phase chromatography columns were tested for their effectiveness in removing unreacted trichloroethanol (TCE) from derivatized samples for gas-liquid chromatography analysis. Derivatized acidic chloroform extracts of saponified whole cells of Mycobacterium species, spent culture media, and derivatized acidic chloroform extracts of serum and cerebrospinal fluids from patients...