Automated Amino Acid Analysis with Sensitive Fluorescence Detection
نویسندگان
چکیده
منابع مشابه
Analysis of plasma amino acids by HPLC with photodiode array and fluorescence detection.
BACKGROUND Plasma amino acids are usually analyzed by ion-exchange chromatography (IEC), a reproducible but time consuming method. Here, we test whether plasma amino acids can be analyzed using reverse-phase high performance liquid chromatography (HPLC). METHODS Filtered plasma, with S-carboxymethyl-l-cysteine as the internal standard, was derivatized and analyzed by an Agilent 1100 HPLC syst...
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Overview Purpose: To develop fast and sensitive approaches for the analysis of underivatized free amino acids and peptides using the latest UHPLC column technology and charged aerosol detection. Methods: Presented here are two distinct methods using the same UHPLC analytical column. The first method uses large amounts of ion-pairing agents to resolve 18 free amino acids. The second method uses ...
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Amino acid analysis (AAA) is one of the best methods to quantify peptides and proteins. Two general approaches to quantitative AAA exist, namely, classical postcolumn derivatization following ion-exchange chromatography and precolumn derivatization followed by reversed-phase HPLC (RP-HPLC). Excellent instrumentation and several specific methodologies are available for both approaches, and both ...
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Ascorbic acid is an important antioxidant that plays an essential role in the biosynthesis of numerous bioactive substances. The detection of ascorbic acid has traditionally been achieved using high-performance liquid chromatography and absorption spectrophotometry assays. However, the development of fluorescence probes for this purpose is highly desired because they provide a much more conveni...
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در این پروژه هدف طراحی و ساخت fluorescence detection systemجهت آشکارسازی مولکول های dna می باشد. در روش pcr، طی چندین چرخه دمایی، مولکول های dna کپی می شوند و سپس با استفاده از روش fluorescence detection، مولکول های بر انگیخته شده dna آشکارسازی می شوند. پس از آشکارسازی می توان به کپی و یا عدم کپی dnaها پی برد.می توان برای جداسازی مولکول های dna بر اساس طولشان، از روش gel electrophoresis استفاده...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1976
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1976.14.1-12.361