Automated degenerate PCR primer design for high-throughput sequencing improves efficiency of viral sequencing
نویسندگان
چکیده
منابع مشابه
Primer design for PCR and sequencing in high-throughput analysis of SNPs.
To achieve high-throughput analysis of allele frequencies in human SNPs, we have developed automated methodsfor designing PCR and DNA sequencing primers. We found we could run the PCR assays at quite stringent, uniform conditions. The design process used freely available databases, including dbSNP, SNPper, and TSC, and publicly available software including RepeatMasker and Primer3. We describe ...
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ژورنال
عنوان ژورنال: Virology Journal
سال: 2012
ISSN: 1743-422X
DOI: 10.1186/1743-422x-9-261