Automated image cytometry for detection of rare, viral antigen-positive cells in peripheral blood

Immunophenotyping of Immune Cells in Peripheral Blood of TB Patient with Positive Smear Before Treatment

ABSTRACT Regarding cellular immunity function and macrophage system in tuberculosis infection control and also tuberculosis high spread in Gilan province and as many studies about tuberculosis has done in chronic and acute progressive process. This study was examined thirty persons smear positive with clinical symptoms and recognition infections expert before Treatment. Tuberculosis patients ...

Signal Amplification of FISH for Automated Detection Using Image Cytometry

The purpose of this study was to improve the detection of FISH signals, in order that spot counting by a fully automated image cytometer be comparable to that obtained visually under the microscope. Two systems of spot scoring, visual and automated counting, were investigated in parallel on stimulated human lymphocytes with FISH using a biotinylated centromeric probe for chromosome 3. Signal ch...

Detection of Intracellular Factor VIII Protein in Peripheral Blood Mononuclear Cells by Flow Cytometry

Flow cytometry is widely used in cancer research for diagnosis, detection of minimal residual disease, as well as immune monitoring and profiling following immunotherapy. Detection of specific host proteins for diagnosis predominantly uses quantitative PCR and western blotting assays. In this study, we optimized a flow cytometry-based detection assay for Factor VIII protein in peripheral blood ...

Novel Automated Method for Minirhizotron Image Analysis: Root Detection using Curvelet Transform

In this article a new method is introduced for distinguishing roots and background based on their digital curvelet transform in minirhizotron images. In the proposed method, the nonlinear mapping is applied on sub-band curvelet components followed by boundary detection using energy optimization concept. The curvelet transform has the excellent capability in detecting roots with different orient...

Contribution of Peripheral Blood CD34-positive Cells for Ligament Healing