Automated projection spectroscopy (APSY)
نویسندگان
چکیده
منابع مشابه
Automated projection spectroscopy and its applications.
This chapter presents the NMR technique APSY (automated projection spectroscopy) and its applications for sequence-specific resonance assignments of proteins. The result of an APSY experiment is a list of chemical shift correlations for an N-dimensional NMR spectrum (N≥3). This list is obtained in a fully automated way by the dedicated algorithm GAPRO (geometric analysis of projections) from a ...
متن کاملA six-dimensional alpha proton detection-based APSY experiment for backbone assignment of intrinsically disordered proteins.
Sequence specific resonance assignment is the prerequisite for the NMR-based analysis of the conformational ensembles and their underlying dynamics of intrinsically disordered proteins. However, rapid solvent exchange in intrinsically disordered proteins often complicates assignment strategies based on HN-detection. Here we present a six-dimensional alpha proton detection-based automated projec...
متن کاملAutomated Resonance Assignment of Proteins: 6 DAPSY-NMR
The 6-dimensional (6D) APSY-seq-HNCOCANH NMR experiment correlates two sequentially neighboring amide moieties in proteins via the C' and Calpha nuclei, with efficient suppression of the back transfer from Calpha to the originating amide moiety. The automatic analysis of two-dimensional (2D) projections of this 6D experiment with the use of GAPRO (Hiller et al., 2005) provides a high-precision ...
متن کاملPrinciples and applications of GFT projection NMR spectroscopy.
The two defining features of G-matrix Fourier transform (GFT) projection NMR spectroscopy are (i) repeated joint sampling of several indirect chemical shift evolution periods of a multidimensional NMR experiment so that transfer amplitudes are generated which are proportional to all possible permutations of cosine and sine modulations of the individual shifts, and (ii) linear combination of the...
متن کاملFast Multidimensional NMR Spectroscopy by the Projection – Reconstruction Technique
16 Spectroscopy 19(10) October 2004 Great strides were made in high-resolution nuclear magnetic resonance (NMR) spectroscopy with the introduction of two-dimensional (2-D) Fourier transformation (1, 2), which spreads the important information into two frequency dimensions. For the first time, spectroscopists were presented with a simple graphical picture of the correlations among different nucl...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2005
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0504818102