Automated Recording of RNA Differential Display Patterns from Pig Granulosa Cells
نویسندگان
چکیده
منابع مشابه
Automated recording of RNA differential display patterns from pig granulosa cells.
We have developed a protocol for fast, nonradioactive, mRNA differential display reverse transcription PCR (DDRT-PCR) based on a commercial automated sequencer with RNA isolated from pig granulosa cells. We sought to discover conditions that would minimize the problem of using relatively small primers labeled with large infrared dye molecule, IR41, required for the sequencer. Extended IR41-labe...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98241rr02