Avirulence gene based RFLP and rep-PCR distinguish the genetic variation of Xanthomonas oryzae pv. oryzae pathotypes in Bangladesh

Bacterial blight (BB) caused by X. oryzae pv. is one of the devastating diseases rice mostly in Asia. Genomes highly variable due to rearrangement large contents transposable elements and dynamic changes population regulated efficiency control measures used for BB management worldwide. In this study, genetic variation pathotypes Bangladesh was studied using aviruelnce gene based RFLP rep-PCR techniques aimed formulate pathogen targeted effective against rice. Eight field isolates were identified on their reactions 10 Near Isogenic Lines (NILs). Among eight pathotypes, IV V contained higher number which 30.13% 23.01% respectively while pathotype VIII revealed as minimum containing only 2.51% total isolates. These avrBs3 repeat domain probe. The results conceded that Bangladeshi strains seem carrying a two maximum nine family genes homologs. resistance phenotype IRBB7 IRBB10 NILs also indicated presence major viz. avrxa7 avrXa10 some pathotypes. Relationship phylogenicity exhibited assorted into haplotypes well these are largely distributed Bangladesh. Phylogenetic analyses carried out (REP, ERIC), BOX depicted main molecular relationship between same lineage possesses different might have originated from common inherited with wide variation.