Azure A embedded in carbon dots as NADH electrocatalyst: Development of a glutamate electrochemical biosensor

Carbon nanodots modified with azure A (AA-CDs) have been synthesized and applied as redox mediator of bioelectrocatalytic reactions. deep characterization AA-CDs nanomaterial has carried out, proving the covalent attachment molecules into carbon dots nanostructure. Disposable screen-printed electrodes (SPCE) AA-CDs, through action chitosan polymer (Chit-AA-CDs/SPCE). The Chit-AA-CDs/SPCE electrocatalytic activity towards oxidation NADH proved, obtaining excellent results regarding low potential achieved (-0.15 V vs. Ag) detection quantification limits (LOD LOQ) for NADH, 16 53 µM, respectively. developed electrochemical platform construction a glutamate biosensor by immobilizing L-glutamic dehydrogenase (GLDH/Chit-AA-CDs/SPCE). morphology GLDH/Chit-AA-CDs/SPCE was analysed AFM at each different step electrode modification process. resulting biosensing is capable detect enzymatically generated GLDH in presence NAD + . Good analytical parameters were obtained analysis using GLDH/Chit-AA-CDs/SPCE, LOD LOQ 3.3 11 successfully to food biological samples. • Azure electrocatalyst oxidation. disposable enzymatic determination.