Bacillus thuringiensis subsp. aizawai generalized transducing phage 4HD248 : restriction site map and potential for fine-structure chromosomal mapping
نویسندگان
چکیده
منابع مشابه
Draft Genome Sequence of Bacillus thuringiensis subsp. aizawai HD133
We report here the 6,512,057-bp draft genome sequence of Bacillus thuringiensis subsp. aizawai HD133. This strain contains at least 6 cry genes and 13 candidate biosynthetic gene clusters.
متن کاملIsolation and characterization of a novel insecticidal crystal protein gene from Bacillus thuringiensis subsp. aizawai.
Bacillus thuringiensis subsp. aizawai EG6346, a novel grain dust isolate, was analyzed by Southern blot hybridization for its insecticidal crystal protein (ICP) gene profile. Strain EG6346 lacks previously characterized cryIA ICP genes yet does possess novel cryI-related gene sequences. A recombinant genomic plasmid library was constructed for strain EG6346 in Escherichia coli. One recombinant ...
متن کاملRefined, circular restriction map of the Bacillus thuringiensis subsp. israelensis plasmid carrying the mosquito larvicidal genes.
All the genetic elements responsible for the mosquito larval toxicity of Bacillus thuringiensis subsp. israelensis are located on one of its largest plasmids, nicknamed pBtoxis. Two linkage groups (with sizes of about 75 and 55 kb) have previously been mapped partially with respect to SacI and BamHI restriction sites (Ben-Dov et al., 1996), but linking them to a single circular plasmid unambigu...
متن کاملFormation of crystals of the insecticidal proteins of Bacillus thuringiensis subsp. aizawai IPL7 in Escherichia coli.
Escherichia coli JM103 cells harboring expression plasmid pTB1 or pKC6 synthesized the 130- and 135-kilodalton insecticidal proteins, respectively, of Bacillus thuringiensis subsp. aizawai IPL7, and both products accumulated as cytoplasmic inclusion bodies. Amorphous inclusions which contained contaminating proteins, together with the corresponding insecticidal proteins, were formed in cultures...
متن کاملLarge crystal toxin formation in chromosomally engineered Bacillus thuringiensis subsp. aizawai due to σE accumulation.
Seven distinct Bacillus thuringiensis subsp. aizawai integrants were constructed that carried the chitinase (chiBlA) gene from B. licheniformis under the control of the cry11Aa promoter and terminator with and without p19 and p20 genes. The toxicity of B. thuringiensis subsp. aizawai integrants against second-instar Spodoptera litura larvae was increased 1.8- to 4.6-fold compared to that of the...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1996
ISSN: 1350-0872,1465-2080
DOI: 10.1099/13500872-142-6-1409