Bacterial Communities within Gingival Tissues from Periodontal Lesions Compared with Subgingival Plaque

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Bacterial Communities within Gingival Tissues from Periodontal Lesions Compared with Subgingival Plaque

Periodontitis is caused by dysbiosis of subgingival plaque that results in increased bacterial invasion into gingival tissues. Although shifts in subgingival microbiota from healthy to periodontitis have been well characterized, the characteristics of bacterial communities located within gingival tissues have not been studied. To characterize microbiota within the tissues of periodontal lesions...

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Bacterial diversity within the human subgingival crevice.

Molecular, sequence-based environmental surveys of microorganisms have revealed a large degree of previously uncharacterized diversity. However, nearly all studies of the human endogenous bacterial flora have relied on cultivation and biochemical characterization of the resident organisms. We used molecular methods to characterize the breadth of bacterial diversity within the human subgingival ...

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Novel strategy to detect and locate periodontal pathogens: The PNA-FISH technique.

PURPOSE We aim to develop peptic nucleic acid (PNA) probes for the identification and localization of Aggregatibacter actinomycetemcomintans and Porphyromonas gingivalis in sub-gingival plaque and gingival biopsies by Fluorescence in situ Hybridization (FISH). METHODS A PNA probe was designed for each microorganism. The PNA-FISH method was optimized to allow simultaneous hybridization of both...

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Microbial complexes in subgingival plaque.

It has been recognized for some time that bacterial species exist in complexes in subgingival plaque. The purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. Subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 y...

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ژورنال

عنوان ژورنال: Journal of Oral Microbiology

سال: 2017

ISSN: 2000-2297

DOI: 10.1080/20002297.2017.1325217