Bacteriophage genotyping using BOXA repetitive-PCR
نویسندگان
چکیده
منابع مشابه
Real-time PCR genotyping using displacing probes.
Simple and reliable genotyping technology is a key to success for high-throughput genetic screening in the post-genome era. Here we have developed a new real-time PCR genotyping approach that uses displacement hybridization-based probes: displacing probes. The specificity of displacing probes could be simply assessed through denaturation analysis before genotyping was implemented, and the probe...
متن کاملMolecular Genotyping of Acinetobacter baumannii Species Isolated from Patients in Tehran, Iran, by Repetitive Element PCR Fingerprinting
Background & Objective: Acinetobacter baumannii is an opportunistic pathogen with high pathogenic and antibiotic-resistance potential and is also considered as one of the main nosocomial agents, specifically in the intensive care units (ICUs). It is highly important to use molecular biology methods in the epidemiological studies, determine the...
متن کاملEvaluation of genotyping large numbers of Escherichia coli isolates by enterobacterial repetitive intergenic consensus-PCR.
We analyzed a large collection of Escherichia coli isolates typed by enterobacterial repetitive intergenic consensus (ERIC)-PCR with BioNumerics gel analysis software. However, the interexperimental variation of ERIC-PCR caused the computer software to classify repeated isolates as different. ERIC-PCR should not be used as the sole determinant of genetic similarity when typing large numbers of ...
متن کاملEnterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) Genotyping of Escherichia coli Strains Isolated from Different Animal Stool Specimens
Background: Escherichia coli is a commensal-pathogenic organism, which includes a wide range of strains. Despite several advanced molecular-genomic technologies for detecting and identifying different strains of E. coli, Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) technique is ...
متن کاملMultiplex universal genotyping using a modified ARMS-PCR protocol.
DNA mutations underlie the pathology of inherited disease. The spectrum of genetic changes resulting in disease is diverse, often encompassing both point (single-nucleotide) and length (large deletions) mutations: cystic fibrosis (2) and the muscular dystrophies (10) are classic examples. Similarly, mutational events implicated in carcinogenesis include both single nucleotide substitutions and ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Microbiology
سال: 2020
ISSN: 1471-2180
DOI: 10.1186/s12866-020-01770-2