Bacteriophage lambda vector for transducing a cDNA clone library into mammalian cells.
نویسندگان
چکیده
منابع مشابه
Introduction of bacteriophage lambda into cells of Klebsiella aerogenes.
We have shown that a mutation in the cro gene of phage lambda greatly reduces zygotic induction. This observation has allowed us to move this phage on an episome into cells of Klebsiella aerogenes where it grows as well as in cells of Escherichia coli. This technique should allow the introduction of various derivatives of lambda into any organism which is able to receive deoxyribonucleic acid f...
متن کاملImproved generalized transducing bacteriophage for Caulobacter crescentus.
Caulobacter phage phi Cr30T, a temperature-sensitive derivative of the lytic, generalized transducing phage phi Cr30, was isolated as a double temperature-sensitive recombinant in a cross between phi Cr30ts1 and phi Cr30ts2, phi Cr30T mediated generalized transduction of Caulobacter crescentus at frequencies comparable to those of phi Cr30 and eliminated the requirement for irradiation of trans...
متن کاملاستفاده از باکتریوفاژ لامبدا به‌عنوان حامل آپوپتین جهت رسانش موثر آن به درون تومور BT-474 سرطان سینه انسانی
Background: Apoptin is a protein from chicken anemia virus that could induce apoptosis specifically in the cancer cells but it has not any effect in the normal cells. Phage therapy is a novel field of cancer therapy and phage nanobioparticles (NBPs) such as λ phage could be modified to deliver and express genetic cassettes into eukaryotic cells safely in contrast with animal viruses. The bacter...
متن کاملIsolation and characterization of specialized lambda transducing bacteriophage carrying the metBJF methionine gene cluster.
Secondary attachment site lysogens of Deltaatt(lambda)Deltappc-argECBH strains of Escherichia coli with lambdacI857 integrated into the bfe gene (88 min) were isolated. Of 20 such lysogens examined, 2 produce lysates with transducing phage containing the metBJF gene cluster (87 min). Reintroduction of the ppc-argECBH chromosome segment (which lies between the bfe and met genes) into these strai...
متن کاملResolution of the DNA strands of the specialized transducing bacteriophage lambda-h80C 1-857 dargF.
The DNA strands of lambdoid phages with deletions or substitutions of the guanine plus cytosine-rich region in the left arm are not resolvable by complexing with poly UG followed by centrifugation in CsCl. This work describes a completely general procedure for the strand resolution of these phages by hybridization with fragments of separated strands of the parent phage. In particular, resolutio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1985
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.5.5.1136